REDCap


RedCap provides automated export procedures for seamless data download to Excel and continuous statistical packages (SPSS, SAS, Stata, R), as well as a built-in project calendar, a scheduling module, ad hoc reporting tools, and advancing features, such as branching logic, file uploading and calculated fields. REDCap is a secure HIPAA compliant web-based application system for building and managing online research surveys and database.

How to access REDCap

https://www.ccbb.howard.edu/redcap/

Email to REDCap support redcap@howard.edu

Contact

Nana Osafo // nosafo@howard.edu

Collaborations and Partnerships

The Collaborations and Partnerships key activity will promote the facilitation and maintenance of collaboration activities involving researchers at Howard University.

Contact

rcmi@howard.edu

Bundled Services Concierge

Each of the RCMI Core facilities (the Center for Computational Biology & Bioinformatics, the Biomedical Imaging Core, the Bioanalytical-Proteomics Core Facility, and the Outcomes Research
Center) are collaborating to provide investigators bundled services that no one facility can provide alone.

Bundled services is intended to promote scientific innovation by providing cutting-edge technical capabilities that will enhance capacity and scientific productivity as well as encouraging and providing new pathways for collaboration among users of the different core facilities.

Professional Development

Specific Aims
  • Provide professional development seminars and workshops to HU Faculty and post-doctoral fellows
  • Provide mentoring for junior faculty recipients of the RCMI pilot project awards
  • To provide mentoring training for mid-level and senior faculty
Contacts

Pamela Carter-Nolan, Ph.D. // pcarter-nolan@howard.edu

Dexter Lee, Ph.D. // dllee@howard.edu

Christian Dark, Okianer Esq. // okianer.c.dark@law.howard.edu

Team Science

http://teamscience.net/index.html
Solutions to complex problems in the sciences require teams of specialists from diverse backgrounds working across the boundaries of disciplinary silos. The COALESCE project aims to create, evaluate, and disseminate new, durable, readily accessible on-line learning resources to enhance skills needed to perform transdisciplinary, team-based translational research. Diverse audiences, including senior investigators, junior investigators, and institutional development officers can benefit from tools designed to help envision how transdisciplinary collaboration can work and overcome the inevitable communication challenges that arise when working in multidisciplinary teams. All content presented in the modules is grounded in empirical research and theory about the science of team science (SciTS), and the experts interviewed are well-published in that domain. The four modules are intended to help researchers acquire and apply a basic knowledge of team science. Modules 2-4 afford an experiential learning environment where the researcher can adopt different roles and engage virtually in the challenges of team research.

Users should complete the free registration and then launch the Science of Team Science Module, then view the outline by navigating within the site http://teamscience.net/activitybrowser/moduleoneoutline.html

Contact

rcmi@howard.edu

Gene Expression Detection

The Gene Expression Detection Core was established in 2019 to examine the levels of gene expressions in cell culture, animals or humans. Generally, two methodologies are usually used to detect the gene expression level: RT-qPCR to detect mRNA or Western blot assay to detect protein. mRNA level stands for the gene transcription while protein level is used to detect translation.

RT-qPCR: a Bio-Rad real time PCR equipment is used to perform the PCR. Total RNA samples should be made by any methods. An RNA gel should be run to check the RNA quality: if the 28S, 18S and 5S rRNA bands are sharp, the RNase contamination can be excluded. In additon, total RNA should be quantitated by dotdrop or OD260/230.

Different kits can be applied, but we recommend using the Bio-rad kit. The detailed protocol can be seen in Bio-Rad website or consulted to our lab persons (Dr. Najealicka Armstrong or Ms. Ruth Crz-cosme).

Following materials are needed: PCR tubes (please check with Ruth before purchasing), PCR kit, primers… a zip drive is needed after experiments.

Western blot : The Core utilizes iBright Western Blot Imaging System for detecting protein levels from cell culture, animal organs or tissue and clinical samples, which stands for gene regulation at a translational level. Current capabilities include the support detection of proteins by western blot and Co-IP assay.

Protocols of the western blot and coIP assay:

Western blot analysis. Proteins were separated by sodium dodecyl sulfate-7.5% polyacrylamide gel electrophoresis (57) (10 to 20 µg loaded in each lane), transferred to nitrocellulose membranes (Amersham Inc., Piscataway, NJ), and blocked with 5% nonfat milk for 60 min at room temperature. Membranes were incubated overnight at 4°C with primary antibody followed by incubation with a horseradish peroxidase-coupled secondary antibody (Amersham Inc.) and detection with enhanced chemiluminescence (Pierce, Rockford, Ill.), according to standard methods. Membranes were stripped with stripping buffer (100 mM β-mercaptoethanol, 2% SDS, 62.5 mM Tris-HCl, pH 6.8), washed with PBS-0.1% Tween 20, and used to detect additional proteins.

Co-Immunoprecipitation (Co-IP) assay. The whole cells were lysed with a lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol) supplemented with protease inhibitor cocktail (cat# p8340, Sigma) on ice for 10 min. The lysates were then centrifuged at 3,000 g for 5 min and the supernatants were transferred to new tubes. The supernatants were incubated with antibodies overnight in a cold room with rolling. The incubations were coupled to protein G-Sepharose beads (Amersham Pharmacia Biotech AB, Sweden) according to the manufacturer's instructions for 3 hours. The beads were washed three time in PBS–0.1% bovine serum albumin and resuspended in a mixture of PBS and 2× Laemmli buffer (20 μl of each). After heating at 95°C for 5 min, beads were removed by centrifugation and supernatants were analyzed by SDS-PAGE and immunoblotting.

For western blot assays, detailed reagents needed to be prepared:
PAGE gel, running buffer, transferring reagents, primary antibody, secondary antibodies, exposure reagents.
For detailed information, please contact Ruth Cruz-cosme.

Contact

Ruth Cruz-cosme: lab manager
ruth.cruzcosme@Howard.edu

Qiyi Tang, Ph.D.
Core Director
Qiyi.tang@howard.edu

The Core utilizes iBright Western Blot Imaging System for detecting protein levels from cell culture, animal organs or tissue and clinical samples. Current capabilities include the detection of proteins by western blot assay only.

Director

Qiyi Tang, Ph.D. // qiyi.tang@howard.edu

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